Journal: The Journal of Clinical Investigation

Article Title: Reprogramming dysfunctional CD8 + T cells to promote properties associated with natural HIV control

doi: 10.1172/JCI157549

Figure Lengend Snippet: Total CD8 + T cells from individuals without HIV were treated with medium, vehicle (Veh.) control, or the GSK3 inhibitor (inh), followed by incubation under basal conditions or with anti-CD3/anti-CD28 stimulation for 48 hours. ( A and B ) Analysis of CD8 + T cell subpopulations in unstimulated cells ( n = 4). ( C ) Fold change of CD8 + T cell subpopulations upon vehicle control or GSK3 inhibitor treatment, relative to the medium alone condition ( n = 4). ( D ) Expression of TCF-1 in CD8 + T cell subsets ( n = 4). ( E ) Fold change in the expression of the indicated markers induced by anti-CD3/anti-CD28 antibody stimulation relative to unstimulated cells ( n = 5). ( F ) Analysis of dead cells by Aqua LIVE/DEAD + staining (Aqua L/D) among total and T-bet + CD8 + T cells, and fold change in dead CD8 + T cells induced by anti-CD3/anti-CD28 stimulation relative to the unstimulated (Unstim.) condition ( n = 5). ( G ) Frequencies of granzyme B + (GZMB + ), IL-2 + , IFN-γ + , and TNF-α + CD8 + T cells after anti-CD3/anti-CD28 stimulation. ( H ) Expression of 1 to 4 functions in CD8 + T cells ( n = 5). * P < 0.05, by Dunn’s test ( B ) and Wilcoxon test ( C , D , and F – H ). Data obtained from 2 ( A – F ) or 3 ( G and H ) independent experiments are shown.

Article Snippet: Purified CD8 + T cells were stained with the LIVE/DEAD Fixable Aqua Dead Cell Stain kit and the following antibodies: anti–CD3 Alexa Fluor 700, anti–CD8 APC Cy7, anti–CCR7 PE Cy7, anti–CD45RA BV421, and anti–CD27 PE (all from BD Biosciences).

Techniques: Incubation, Expressing, Staining

Journal: Journal of vascular research

Article Title: Mitochondrial Mechanisms in Cerebral Vascular Control: Shared Signaling Pathways with Preconditioning

doi: 10.1159/000360765

Figure Lengend Snippet: Mitochondrial morphology within a cerebral vascular endothelial cell. The mitochondria (red) and the nuclei (blue) of live rat brain microvascular endothelial cells were stained using mitochondrial stain (1:10000; lex: 514 nm and lem: 538-681 nm) and Hoechst nuclear stain (1: 1000; lex: 405 nm and lem: 410-585 nm), respectively (ChromeoTM Live Cell Mitochondrial Staining kit, Active Motif, Carlsbad, California). Fluorescence images were obtained using LSM 710 AxioObserver (Carl Zeiss, Hamburg, Germany) with plan-Apochromat 63×/Numerical Aperture 1.4 oil immersion objective.

Article Snippet: The mitochondria (red) and the nuclei (blue) of live rat brain microvascular endothelial cells were stained using mitochondrial stain (1:10000; l ex : 514 nm and l em : 538-681 nm) and Hoechst nuclear stain (1: 1000; l ex : 405 nm and l em : 410-585 nm), respectively (ChromeoTM Live Cell Mitochondrial Staining kit, Active Motif, Carlsbad, California).

Techniques: Staining, Fluorescence

Journal: Light, Science & Applications

Article Title: Phasor-FSTM: a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing

doi: 10.1038/s41377-024-01711-y

Figure Lengend Snippet: a Unresolved confocal images at different imaging depths (z-step: 0.5 μm) in a HeLa cell. b 3D z-stack of confocal images in panel a before unmixing (different colors indicate different depths). c Fluorescence decay curves of the original FLIM data. d Phasor plots change with the imaging depth . e Comparison of two-color confocal and Phasor-FSTM in terms of 3D z-stack images. SBRs were calculated to demonstrate the improvement in image quality of the proposed method compared to confocal. f Normalized intensity profiles along the arrows in the enlarged images of e . g Mean FWHM values of the intensity profiles across the structures at five positions. Scale bars, 5 μm ( a, b, e )

Article Snippet: We further demonstrated two-color phasor-FSTM by imaging lysosomes and microtubules in live HeLa cells using another two kits (TraKineTM Pro Live-cell Lysosome Staining Kit, Abbkine; Cell NavigatorTM Live Cell Tubulin Staining Kit, AAT Bioquest).

Techniques: Imaging, Fluorescence, Comparison

Journal: Light, Science & Applications

Article Title: Phasor-FSTM: a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing

doi: 10.1038/s41377-024-01711-y

Figure Lengend Snippet: a Confocal image of a single HeLa cell consisting of the mitochondrion (MitoTracker Deep Red FM), microtubule (Tubulin Tracker Deep Red), lysosome (LysoBrite NIR), and nucleus (Nuclear Red LCS1) structures. b FSTM image without the phasor process. c Four-color phasor-FSTM super-resolution images before (top right) and after (lower left) contrast adjustment. Mitochondrion: yellow; microtubule: green; lysosome: magenta; nucleus: cyan. d Magnified views of the white squares in a–c . e Photon number distributions in original diffraction-limited and super-resolution images, and intensity profiles in one-color and four-color super-resolution images post-deconvolution, plotted along the dotted lines in d

Article Snippet: We further demonstrated two-color phasor-FSTM by imaging lysosomes and microtubules in live HeLa cells using another two kits (TraKineTM Pro Live-cell Lysosome Staining Kit, Abbkine; Cell NavigatorTM Live Cell Tubulin Staining Kit, AAT Bioquest).

Techniques:

Journal: iScience

Article Title: αβ-T cell receptor transduction gives superior mitochondrial function to γδ-T cells with promising persistence

doi: 10.1016/j.isci.2023.107802

Figure Lengend Snippet:

Article Snippet: Live or dead fixable dead cell staining kit NIR fluorescence , AAT Bioquest , Cat#22605.

Techniques: Recombinant, Staining, Fluorescence, Electrophoresis, Mass Spectrometry, Microarray, Software